Use of glucose analogues to study the mechanism of glucose‐mediated cAMP increase in yeast

FEBS Letters

Gancedo, Juana M.¹  Eraso, Pilar¹ 

[1] Departamento de Bioquimica de la Facultadde Medicina de la Universidad Autónoma de Madrid and Institute de Investigaciones Biomedicas del CSIC Arzobispo Morcillo 4, 28029 Madrid, Spain

关键词: cAMP;    Yeast;    Non-metabolizable sugar;    Fructose-1;    6-bisphosphatase;    Phosphorylation;    Physiological inactivation;   

DOI  :  10.1016/0014-5793(85)80991-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The addition of glucose to yeast triggers a transient, several-fold increase in cAMP levels followed by a permanent 2-fold increase. The transient increase can be provoked by a variety of glucose analogues, even those that cannot be phosphorylated, and also by galactose. The extent of the increase appears to be correlated with the affinity of the transport system for the different sugars. The permanent increase in cAMP occurs only with sugars that can be efficiently phosphorylated. The maximal cAMP concentration reached after addition of the sugars determines the extent of inactivation of fructose-1,6-bisphosphatase.

【 授权许可】

Unknown