Novel structure of heat shock messenger ribonucleoproteins in Drosophila Kc cells

FEBS Letters

Köhler, Kurt¹  Setyono-Han, Buddy¹ 

[1] Biologisches Institut der Universität Stuttgart, D-7000 Stuttgart 60, FRG

关键词: Drosophila;    Polyribosomal mRNP;    Heat shock messenger;    Poly (A)-protein;    UV cross-linking;   

DOI  :  10.1016/0014-5793(85)80990-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Poly(A)⁺ polyribosomal mRNA is normally complexed with a specific set of proteins forming ribonucleoprotein particles, termed polyribosomal mRNPs. We have found that in Drosophila during the heat shock response, their structure is profoundly altered as shown by their greatly decreased density in Cs₂SO₄ gradients. RNA-protein cross-linking, conducted in vivo, revealed that polyribosomal mRNA is associated with only two polypeptides of 70 and 38 kDa; while those from cells raised at 25°C revealed a more complex protein pattern. The 38 kDa protein is located on the poly(A) sequences.

【 授权许可】

Unknown