A new isocratic HPLC separation for Pth‐amino acids, based on 2‐propanol

FEBS Letters

Ashman, Keith¹  Wittmann-Liebold, Brigitte¹ 

[1] Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Ihnestrasse 63-73, D-1000 Berlin 33 (Dahlem), Germany

关键词: Phenylthiohydantoin amino acid;    2-Propanol;    Tetrahydrofuran;    Isocratic separation;    HPLC;   

DOI  :  10.1016/0014-5793(85)80442-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The described isocratic separation for phenylthiohydantoin (Pth) amino acid derivatives offers advantages over gradient systems: the baseline is more stable at high sensitivity, the resolution and reproducibility is excellent and the cost and consumption of solvent is very low. The separation of low picomolar quantities, on 2 different types of reverse phase columns, are presented. The method is used ‘off line’, i.e. as a separate analytical unit, or in an ‘on line’ mode, where it is directly connected to a protein sequencer for automatic identification of the released amino add derivatives.

【 授权许可】

Unknown