Sympathetic neurons extend neurites in a culture medium containing cyanide and dinitrophenol but not iodoacetate

FEBS Letters

Wakade, Arun R.¹  Wakade, Taruna D.¹  Prat, John C.¹ 

[1] Department of Pharmacology, State University of New York, Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA

关键词: Neuronal culture;    Neuronal metabolism;    ATP;    Cyanide;    Glycolysis;    Nerve growth factor;   

DOI  :  10.1016/0014-5793(85)80435-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A culture medium circulated through the rat heart and supplemented with insulin, transferrin and nerve growth factor leads to a massive proliferation of neurite outgrowth from neurons of peripheral sympathetic ganglia of the chick embryo. Addition of 1 mM cyanide or 50 μM dinitrophenol to such medium for 2 days had no adverse effect on the neurite outgrowth and ATP content of these neurons. However, 0.5 μM iodoacetate lowered ATP content 65% without affecting the number of surviving neurons up to 2 days. Only when ATP content was reduced to 80% by 2.5 μM iodoacetate was the number of surviving neurons significantly reduced (30%). It is concluded that the glycolytic pathway is the major route of ATP synthesis in embryonic sympathetic neurons maintained in culture, and only a small fraction of ATP is utilized for the survival and neurite extension.

【 授权许可】

Unknown