Isolation and amino acid sequence of the smallest subunit of beef heart bc 1 complex

FEBS Letters

Aquila, Heinrich¹  von Jagow, Gebhard¹  Schägger, Hermann¹  Link, Thomas A.¹  Borchart, Ursula¹ 

[1] Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität, Goethestr. 33, 8000 München 2, FRG

关键词: Cytochrome bc1 complex;    SDS-PAGE system;    Amino acid sequence;    Smallest suburit;    Membrane protein;    Hydroxyapatite chromatography;   

DOI  :  10.1016/0014-5793(85)80434-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 11 subunits of beef heart bc ₁ complex can be separated either by a new SDS-PAGE system or by a series of Chromatographic steps involving dissociation of the complex by salt treatment. The amino acid sequence of the smallest subunit was determined by complete solid-phase Edman degradation and was confirmed by sequencing the N-terminal part and the C-terminal tryptic fragment by liquid-phase Edman degradation. The protein consists of 56 amino acid residues; the M _r was calculated to be 6363. The protein (‘ISP binding factor’) might be entangled in the reassembly of the iron-sulfur protein with the bc ₁ subcomplex.

【 授权许可】

Unknown