Phorbol myristate acetate causes in guinea‐pig lung parenchymal strip a maintained spasm which is relatively resistant to isoprenaline

FEBS Letters

Dale, M.Maureen¹  Obianime, W.¹ 

[1] Department of Pharmacology, University College London, Gower Street, London WC1E 6BT, England

关键词: Polyphosphoinositide;    Phorbol ester;    Smooth muscle;    Asthma;    Protein kinase;    PMA;    phorbol myristate acetate;    DMSO;    dimethyl sulphoxide;   

DOI  :  10.1016/0014-5793(85)80415-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of phorbol myristate acetate (PMA) was compared with that of histamine on the guinea-pig lung parenchymal strip. PMA, 10⁻⁵ M, caused a slowly developing sustained contraction which had approximately the same magnitude as the maximal histamine contraction. Isoprenaline, at 10⁻⁵ M, caused 86% relaxation of the histamine contraction but only 22% relaxation of the PMA contraction. Forskolin, at 10⁻⁵ M had a similar action to isoprenaline on the effects of both spasmogens while aminophylline, 5 × 10⁻⁴ M, was considerably less effective. Sodium nitroprusside had little effect on the histamine contraction and actually increased the PMA spasm. It is suggested that protein kinase C may have a role in the tonic phase of the contraction of bronchiolar smooth muscle. These findings could have relevance for the delayed phase of asthma, which is known to be insensitive to β-agonists.

【 授权许可】

Unknown