FEBS Letters | |
The release of polypeptides and manganese from oxygen‐evolving photosystem II preparations following zinc‐treatment | |
Miller, Mette1  | |
[1] Institute of Biochemistry, Odense University, Campusvej 55, 5230 Odense M, Denmark | |
关键词: Photosystem II; Oxygen evolution; Manganese; Polypeptide; | |
DOI : 10.1016/0014-5793(85)81055-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Inhibition of oxygen evolution in photosystem II membrane fragments from pea chloroplasts by washing with Zn2+ causes appearance of the EPR signal of Mn(H2O)6 2+. This Mn2+ remains associated with the membrane fraction. Release of Mn2+ into the medium was correlated with the amount of the 23 kDa protein removed from the membrane. This suggests that this protein may function as a ‘gate’ to an aqueous compartment into which Mn2+ is released. Inhibition by Zn2+ correlated with the release of 1 Mn2+ per reaction centre, out of a total stoichiometry of 4 Mn atoms per reaction centre. By comparing the release of Mn following Zn-treatment of NaCI or CaC12 washed membranes, it is concluded that the 33 kDa protein is involved in binding of 2 Mn.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020287163ZK.pdf | 537KB | download |