The release of polypeptides and manganese from oxygen‐evolving photosystem II preparations following zinc‐treatment

FEBS Letters

Miller, Mette¹ 

[1] Institute of Biochemistry, Odense University, Campusvej 55, 5230 Odense M, Denmark

关键词: Photosystem II;    Oxygen evolution;    Manganese;    Polypeptide;   

DOI  :  10.1016/0014-5793(85)81055-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Inhibition of oxygen evolution in photosystem II membrane fragments from pea chloroplasts by washing with Zn₂₊ causes appearance of the EPR signal of Mn(H₂O)₆ ²⁺. This Mn²⁺ remains associated with the membrane fraction. Release of Mn²⁺ into the medium was correlated with the amount of the 23 kDa protein removed from the membrane. This suggests that this protein may function as a ‘gate’ to an aqueous compartment into which Mn²⁺ is released. Inhibition by Zn²⁺ correlated with the release of 1 Mn²⁺ per reaction centre, out of a total stoichiometry of 4 Mn atoms per reaction centre. By comparing the release of Mn following Zn-treatment of NaCI or CaC1₂ washed membranes, it is concluded that the 33 kDa protein is involved in binding of 2 Mn.

【 授权许可】

Unknown