Antibodies directed against guanylic acid and guanosine are nucleolus specific probes

FEBS Letters

Krishnamurthy, D.S.¹  Jacob, T.M.²  Iswari, S.² 

[1] Department of Zoology, Schoolof Life Sciences, Gujarat University, Ahmedabad 380009, India;Department of Biochemistry and ICMR Centre for Cell Biology and Genetics, Indian Institute of Science, Bangalore, 560012 India

关键词: Indirect immunofluorescence;    Guanylic acid antibody;    Human lymphocyte;    Guanosine binding;    dsDNA;    double-stranded DNA;    EDC;    1-ethyl-3-(3-dimethylisopropyl)carbodiimide;    FITC;    fluorescein-isothiocyanate;    Gox-red;    guanosine oxidized with NaIO4 and reduced with NaBH4 (also for other nucleosides);    G-bands;    Geimsa bands;    IgG;    immunoglobulin-G;    pG;    guanylic acid;    Q-bands;    quinacrine bands;    R-bands;    reverse bands;    ssDNA;    single-stranded DNA;    γ-G;    γ-globulins;   

DOI  :  10.1016/0014-5793(85)81043-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Purified antibodies against guanylic acid and guanosine binding to RNA at guanosine residues were used to probe human lymphocyte preparations by indirect immunofluorescence. Neither antibody gave any banding pattern with metaphase chromosomes but both showed binding to specific sites in the interphase nuclei. Evidence presented indicates that these sites are guanosine residues on rDNA transcripts at the nucleolar organizer regions.

【 授权许可】

Unknown