Wedeloside, a powerful inhibitor and ligand of the mitochondrial ADP/ATP carrier

FEBS Letters

Appel, Maria¹  Klingenberg, Martin¹  Oelrichs, Peter B.¹ 

[1] Institut für Physikalische Biochemie der Universität München, Goethestrasse 33, 8000 München 2, FRG

关键词: Wedeloside;    Mitochondria;    ADP/ATP carrier;    Inhibition;    ATR;    atractylate;    CAT;    carboxyatractylate;    WED;    wedeloside;   

DOI  :  10.1016/0014-5793(85)81032-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of wedeloside, an atractyloside analogue occurring in the Australian weed Wedelia asperrima, on the ADP/ATP carrier is investigated on 3 levels; the ADP stimulated respiration, the ADP/ATP exchange, and the binding in competition with atractylate (ATR) and carboxyatractylate (CAT). The inhibition of respiration and of ADP/ATP exchange by wedeloside is nearly uncompetitive with ADP. The competitive binding with [³H]cat and [³H]ATR reveals a high binding affinity of wedeloside similar to that of CAT. The titration of the ADP/ATP exchange and of the binding with wedeloside gives a titer of 0.35 protein for rat liver protein and the titer of the binding gives 2.7 protein for beef heart mitochondria. The 1.8-times higher titers than with CAT may indicate that two molecules of wedeloside bind to one ADP/ATP carrier dimer in contrast to the half site reactivity known for the binding of the other ligands.

【 授权许可】

Unknown