Modification of mammalian fatty acid synthetase activity by NADP

FEBS Letters

Smith, Stuart²  Linn, Tracy¹  Stern, Alan² 

[1] Veterans Administration Hospital, Dallas, TX 75216, USA;Research Laboratory, Children's Hospital Medical Center, Oakland, CA 94609 USA

关键词: Fatty acid synthetase;    NADP;    Acetoacetyl-enzyme;   

DOI  :  10.1016/0014-5793(85)81029-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Experiments are described which show that the mammalian fatty acid synthetase, in the presence of NADP, synthesizes stoichiometric amounts of enzyme-bound acetoacetyl moieties. The acetoacetyl moieties can neither undergo the normal transfer reaction to a CoA acceptor, nor participate in the normal reaction sequence once NADPH is made available. Our results indicate that, since it is the product of the condensation reaction which accumulates on the inhibited enzyme, the previously held view that NADP inhibits the condensation step in fatty acid synthesis is probably incorrect.

【 授权许可】

Unknown