β‐Granins: 21 kDa co‐secreted peptides of the insulin granule closely related to adrenal medullary chromogranin A

FEBS Letters

Hutton, John C.¹  Hansen, Frede²  Peshavaria, Mina¹ 

[1] Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge, England;TheUniversity Department of Clinical Chemistry, Rigshospitalet, Copenhagen, Denmark

关键词: Pancreas;    Insulin;    Chromaffin;    Secretory granule;   

DOI  :  10.1016/0014-5793(85)80398-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Three closely related forms of a 21 kDa protein which is co-secreted with insulin have been purified and analysed. These differed in behaviour on ion-exchange chromatography but were indistinguishable by their susceptibility to staphylococcal V8 proteinase digestion, amino acid composition or N-terminal amino acid sequence. Their amino acid composition and N-terminal sequences were remarkably similar to adrenal medullary chromogranin A, a much larger protein (72 kDa). Antibodies to chromogranin A also reacted strongly with the 21 kDa protein in isolated insulin granules. It is concluded that the 21 kDa proteins either represent a repeated domain within the chromogranin molecule or a closely related gene product. The name β-granin is proposed for these proteins.

【 授权许可】

Unknown