Vasoactive intestinal peptide receptor activity and specificity during enterocyte‐like differentiation and retrodifferentiation of the human colonic cancerous subclone HT29‐18

FEBS Letters

Rosselin, Gabriel¹  Gespach, Christian¹  Chastre, Eric¹  Emami, Shahin¹ 

[1] INSERM U.55, Hôpital Saint-Antoine, 184, rue du Fg. St. Antoine, 75571 Paris Cedex 12, France

关键词: HT29-18 subclone;    Enterocyte-like differentiation;    VIP receptor activity;    Adenylate cyclase;    Forskolin;   

DOI  :  10.1016/0014-5793(85)80371-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Commitment of HT29-18 cells to enterocyte-like differentiation by glucose removal is related to a decreased capacity to generate cAMP after treatment with vasoactive intestinal peptide (VIP), forskolin or sodium fluoride. In contrast, the potency of VIP (EC₅₀ = 1.1–1.3 × 10⁻¹⁰ M) and the pharmacological specificity of the VIP receptor (VIP > rh GRF 1 -43 > PHI > secretin) are unchanged during differentiation and retrodifferentiation. These results indicate that disturbances in VIP receptor-post-receptor activity, involving cell surface VIP receptors, membrane and intracellular transducers of hormonal information, occur during enterocyte-like differentiation of the HT29-18 subclone.

【 授权许可】

Unknown