FEBS Letters | |
Vasoactive intestinal peptide receptor activity and specificity during enterocyte‐like differentiation and retrodifferentiation of the human colonic cancerous subclone HT29‐18 | |
Rosselin, Gabriel1  Gespach, Christian1  Chastre, Eric1  Emami, Shahin1  | |
[1] INSERM U.55, Hôpital Saint-Antoine, 184, rue du Fg. St. Antoine, 75571 Paris Cedex 12, France | |
关键词: HT29-18 subclone; Enterocyte-like differentiation; VIP receptor activity; Adenylate cyclase; Forskolin; | |
DOI : 10.1016/0014-5793(85)80371-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Commitment of HT29-18 cells to enterocyte-like differentiation by glucose removal is related to a decreased capacity to generate cAMP after treatment with vasoactive intestinal peptide (VIP), forskolin or sodium fluoride. In contrast, the potency of VIP (EC50 = 1.1–1.3 × 10−10 M) and the pharmacological specificity of the VIP receptor (VIP > rh GRF 1 -43 > PHI > secretin) are unchanged during differentiation and retrodifferentiation. These results indicate that disturbances in VIP receptor-post-receptor activity, involving cell surface VIP receptors, membrane and intracellular transducers of hormonal information, occur during enterocyte-like differentiation of the HT29-18 subclone.
【 授权许可】
Unknown
【 预 览 】
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