Preparation and amino acid sequence of human κ‐casein

FEBS Letters

Ribadeau-Dumas, Bruno¹  Brignon, Ghislaine¹  Chtourou, Abdessatar¹ 

[1] Institut National de la Recherche Agronomique, CNRZ, 78350 Jouy-en-Josas, France

关键词: k-Casein;    Primary structure;    Protein evolution;    Human;   

DOI  :  10.1016/0014-5793(85)80872-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human κ-casein was prepared from whole casein by successive hydroxyapatite and thiol-Sepharose chromatographies. The primary structure of its 99-residue N-terminal fragment has been determined by sequencing peptides obtained by tryptic and chymotryptic digestions of the whole protein. This fragment overlaps the known sequence of the 65-residue C-terminal fragment. The 158-residue sequence of human κ-casein was compared to those of goat, ewe, cow and rat κ-caseins. Only 22% of the residues are identical in homologous positions. The rate of divergence of the 93-residue N-terminal segment (para-κ-casein) appears to be higher than that of the rest of the molecule.

【 授权许可】

Unknown