A fluorescence study of the ternary system lecithin‐sodium deoxycholate‐water showing structural transformations

FEBS Letters

Naplatarova, Maria D.¹  Stoylov, Stoyl P.¹  Antonov, Peter A.¹  Pancheva, Rayna P.¹ 

[1]Department of Physics and Biophysics, Scientific Medico-Biological Institute, Medical Academy, 1431 Sofia, Bulgaria

关键词: Ternary system;    Structural transformation;    Fluorescence method;    Rhodamine-B;   

DOI  :  10.1016/0014-5793(85)81269-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A fluorescence method was applied to study structural transitions occurring in the ternary system lecithinsodium deoxycholate-water. The method is based on binding of the hydrophilic probe rhodamine-B to the structures at molar ratios of lecithin and deoxycholate (L/D) ranging from 0.16 to 10.7. The relationship between the fluorescence intensity and L/D ratio was sharply altered at molar ratios of 0.9 and 4.5. Thus three L/D regions could be distinguished and inflection points were estimated for each one. It is suggested that the L/D values of the sharp transitions (0.9 and 4.5) and the inflection points (0.5, 1.5 and 5.5) indicate generation of new types of molecular arrangements in the ternary system.

【 授权许可】

Unknown