The inhibition of mitochondrial F1‐ATPase by 1,5‐difluoro‐2,4‐dinitrobenzene

FEBS Letters

Kozlov, I.A.¹  Tsybovsky, I.S.²  Akhrem, A.A.²  Kisel, M.A.²  Vulfson, E.N.¹ 

[1] A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR;Institute of Bioorganic Chemistry of the Byelorussian Academy of Sciences, Zhodinskaya 5, Minsk 220600 USSR

关键词: Mitochondrial F1-ATPase;    Tyrosine modification;   

DOI  :  10.1016/0014-5793(85)81252-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

1,5-Difluoro-2,4-dinitrobenzene completely inhibits F₁-ATPase when it is used at micromolar concentrations and the inhibitor/enzyme molar ratio is equal to 3. The inhibition can be reversed by dithiothreitol treatment. 7-Chloro-4-nitrobenzofurazan treatment of F₁-ATPase does not prevent the reaction of the enzyme with 1,5-difluoro-2,4-dinitrobenzene. The 1,5-difluoro-2,4-dinitrobenzene-induced inhibition is thought to be a result of the modification of a tyrosine residue with pK 9.1.

【 授权许可】

Unknown