Energy expenditure for cyclic retention of NH3/NH4 + during N2 fixation by Klebsiella pneumoniae

FEBS Letters

Kleiner, D.¹ 

[1] Lehrstuhl für Mikrobiologie, Universität Bayreuth, Postfach 3008, 8580 Bayreuth, FRG

关键词: NH3 cyclic retention;    NH3 permeability;    N2 fixation energetics;   

DOI  :  10.1016/0014-5793(85)81249-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The permeability coefficeint for NH₃ through bacterial membranes was determined to be around 2 × 10⁻³ cm·s⁻¹. This value was used to calculate the outward diffusion of NH₃ from intracellular pools and the energy costs for reabsorption as NH₄ ⁺. For an N₂-fixing continuous culture of Klebsiella pneumoniae an energy expenditure of around 4 ATP per NH₃ produced was calculated, thus increasing significantly the energy requirement for N₂ fixation in vivo.

【 授权许可】

Unknown