Light‐induced Fourier transform infrared (FTIR) spectroscopic investigations of the primary donor oxidation in bacterial photosynthesis

FEBS Letters

Nabedryk, E.²  Kreutz, W.¹  Tavitian, B.A.²  Breton, J.²  Mäntele, W.¹ 

[1] Institut für Biophysik und Strahlenbiologie der Universität Freiburg, Albertstrasse 23, D-7800 Freiburg, FRG;Servicede Biophysique, Département de Biologie, CEN Saclay, 91191 Gif-sur-Yvette Cédex, France

关键词: Fourier transform infrared spectroscopy;    Reaction center;    Bacterial photosynthesis;    Primary donor;    Chlorophyll;    I;    intermediate acceptor;    QA;    QB;    primary;    secondary quinone;   

DOI  :  10.1016/0014-5793(85)81247-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fourier transform infrared (FTIR) difference spectroscopy of the primary electron donor (P) photo–oxidation has been performed for reaction centers (RCs) and chromatophores of purple photosynthetic bacteria. In the 1800–650 cm⁻¹ spectral region highly reproducible absorbance changes were obtained that can be related to specific changes of individual bond absorption. Several bands in the difference spectra are tentatively assigned to changes of intensity and position of the keto and ester C−O vibrations of the P bacteriochlorophylls, and a possible interpretation in terms of changes of their environment or type of bonding to the protein is given. Small difference bands in the amide I and II region allow only minor protein conformational changes.

【 授权许可】

Unknown