1H NMR detection of cerebral myo‐inositol

FEBS Letters

Cerdán, Sebastián¹  Santoro, Jorge²  Parrilla, Roberto¹  Rico, Manuel² 

[1] Instituto Gregorio Marañón, CS1C Velázquez 144, 28006 Madrid, Spain;Institute de Estructura de la Materia, CSIC, Serrano 119, 28006 Madrid, Spain

关键词: Cerebral myo-inositol1H NMR quantitative analysis Brain development;    Ac−;    acetate;    BHBA;    β-hydroxybuty-rate;    Cr;    creatine;    CrP;    creatine phosphate;    2D COSY;    bidimensional correlated spectroscopy;    FT NMR;    Fourier transform NMR;    GABA;    γ-aminobutyrate;    GPC;    glycerolphosphorylcholine;    Lac;    lactate;    myo-ino;    myo-inositol [(1;    2;    3;    5/4;    6)cyclohexanehexol];    N-Ac-Asp;    N-acetylaspartate;    PC;    phosphorylcholine;    TSP;    2;    2;    3;    3-tetradeutero-3-trimethylsilylpropionate. Abbreviations used for the amino acids are those recommended by the IUPAC-IUB Commission on Biochemical Nomenclature;   

DOI  :  10.1016/0014-5793(85)81235-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A previously unassigned group of prominent multiplets of the 360 MHz ¹H NMR spectrum of acid stable metabolite extracts from rat brain is shown to arise from free myo-inositol. This conclusion is derived from a systematic analysis of the high-resolution ¹H NMR spectra of brain acid extracts, in which appropriate conditions and optimal proton signals have been selected for the quantitative analysis of up to 15 metabolites. Developmental variations in the cerebral content of myo-inositol could be readily detected using this approach, which provides a novel alternative to study myo-inositol metabolism under physiological or pathological conditions.

【 授权许可】

Unknown