On the prosthetic group(s) of component II from nitrogenase

FEBS Letters

Braaksma, A.²  Dunham, W.R.¹  Haaker, H.²  Hagen, W.R.² 

[1] Biophysics Research Division, Institute of Science and Technology, The University of Michigan, Ann Arbor, MI 48109, USA;Department ofBiochemistry, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands

关键词: Nitrogenase Fe-protein A.vinelandii ESR Spin-spin interaction;   

DOI  :  10.1016/0014-5793(85)81231-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The EPR spectrum of the reduced Fe-protein from nitrogenase has been reinvestigated. The dependences on temperature, microwave power, and microwave frequency all suggest that the observed signal represents a magnetically isolated [4Fe-4S]^1+(2+;1+) cluster. Also, the signal can be simulated assuming a simple, gstrained S = system. However, the integrated intensity amounts to no more than 0.2 spins per protein molecule. It is, therefore, impossible that Fe-protein preparations contain a single type of [4Fe-4S] cluster.

【 授权许可】

Unknown