Quantitative measurement of the total, peroxyl radical‐trapping antioxidant capability of human blood plasma by controlled peroxidation

FEBS Letters

Burton, G.W.²  Wayner, D.D.M.²  Ingold, K.U.²  Locke, S.¹ 

[1] Department of Chemistry, Mount Allison University Sackville, New Brunswick EOA 3CO, Canada;Division of Chemistry, National Research Council of Canada Ottawa, Ontario K1A OR6, Canada

关键词: Plasma auto-oxidation Vitamin C Vitamin E Urate Antioxidant Protein;   

DOI  :  10.1016/0014-5793(85)81208-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Plasma obtained from fasted humans has been analysed for total radical-trapping antioxidant content by subjecting it to controlled peroxidation using the thermal decomposition of water-soluble azobis (2-amidinopropane hydrochloride) at 37°C to produce peroxyl radicals at a known, steady rate. It is found that the total radical-trapping antioxidant content is rather similar for the 7 subjects that have been tested and, furthermore, it is 10–20-times larger than the effect attributable to vitamin E alone. Although it is shown that urate and ascorbate augment the contribution from vitamin E, their contributions (21–34 and 0–2%, respectively) still leave 57–73% of the total antioxidant content unaccounted for. Evidence is presented to show that this previously unrecognized large reserve of antioxidant capacity is attributable to the plasma proteins.

【 授权许可】

Unknown