Identification of a calcium‐ and phospholipid‐dependent protein kinase in plant tissue

FEBS Letters

Matzenauer, Sybille¹  Schäfer, Angelika¹  Marmé, Dieter¹  Bygrave, Fyfe¹ 

[1] Institute of Biology III, University of Freiburg, 7800 Freiburg, FRG

关键词: Protein kinase Calcium Phospholipid Protein phosphorylation Plant;   

DOI  :  10.1016/0014-5793(85)81206-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A Ca²⁺- and phospholipid-activated protein kinase from zucchini has been partially purified by DEAE-Sephacel chromatography and some properties of the enzyme have been assessed. Minimal activity occurs in the absence of added Ca²⁺ or of added phospholipid but concentrations of the free ion in the range of 3 × 10⁻⁷ M produce a marked stimulation. At 10⁻⁶ M free Ca²⁺ this activity is further enhanced by phosphatidylserine, phosphatidylethanolamine and phosphatidic acid but not by phosphatidylcholine or phosphatidylinositol. All of these phospholipids and especially phosphatidylinositol stimulate protein kinase activity in the absence of added Ca²⁺.

【 授权许可】

Unknown