Purification of liver and hepatoma membrane proteins by high‐performance liquid chromatography

FEBS Letters

Schuett, W.¹  Neumeier, R.¹  Reutter, W.¹  Josić, Dj.¹ 

[1] Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000 Berlin 33 (Dahlem), Germany

关键词: Liver;    Hepatoma;    Membrane protein;    High-performance liquid chromatography;    HPLC;    high-performance liquid chromatography;    SE;    size exclusion;    RP;    reversed phase;    PBS;    phosphate-buffered saline;    PMSF;    phenylmethyl sulfonyl fluoride;    CHAPS;    3-(3-chloramidopropyl) dimethylammonio-1-propane-sulfonate;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(85)80766-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This paper documents the recovery of selected proteins from hepatic plasma membranes. Initial purification, achieved by a series of stepwise extractions, facilitates the subsequent purification by HPLC. Examples are provided to illustrate the recovery of specific proteins from two Morris hepatoma lines and the liver.

【 授权许可】

Unknown