Treatment of oocyte membranes with the 2',3'‐dialdehyde of guanosine triphosphate reduces progesterone inhibition of adenylyl cyclase

FEBS Letters

Olate, Juan¹  Anker, Roberto¹  Allende, Jorge E.¹ 

[1] Departamento de Bioquímica, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago 7, Chile

关键词: Adenylyl cyclase;    GTP 2';    3'-dialdehyde;    Progesterone inhibition;    Stimulatory submit Ns;    G-protein;    Ns;    stimulatory guanine nucleotide binding component that regulates adenylyl cyclases;    Ni;    inhibitory guanine nucleotide binding component that regulates adenylyl cyclases;    Gpp(NH)p;    guanyl-5'-yl imidodiphosphate;    App(NH)p;    adenyl-5'-yl imidodiphosphate;    dial GTP or dial ATP;    the 2';    3' dialdehyde of the nucleotide triphosphate;   

DOI  :  10.1016/0014-5793(85)80764-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of Xenopus laevis membranes with the 2',3'-dialdehyde of GTP (dial GTP) drastically inhibits their adenylyl cyclase activity. Optimal inhibition is obtained by treatment with 1 mM dial GTP for Ih at 32°C. Using guanyl-5'-yl imidodiphosphate, F⁻, forskolin and Mn²⁺ as activators of the enzyme it can be concluded that dial GTP preferentially reacts with the stimulatory subunit (N_s) and slightly with the catalytic subunit. Dial GTP treatment greatly reduces the inhibition of adenylyl cyclase by progesterone. Pure exogenous N_s stimulates the enzyme but does not restore progesterone inhibition. Treatment with dial [α-³²P]GTP labels several membrane proteins some of which have similar M _r to N_s and N_i.

【 授权许可】

Unknown