Affinity of protein HU for different nucleic acids

FEBS Letters

Holck, Askild¹  Kleppe, Kjell¹ 

[1] Department of Biochemistry, University of Bergen, Bergen, Norway

关键词: Chromatography;    Affinity;    Bacterial protein;    DNA binding;   

DOI  :  10.1016/0014-5793(85)80753-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding of protein HU from Escherichia coli to nucleic acids was investigated by affinity chromatography under various conditions, by a nitrocellulose retention assay and by isopycnic centrifugations in metrizamide gradients. The results indicate that HU has a preference for binding to RNA and single-stranded DNA over double-stranded DNA. The affinity of HU for supercoiled DNA was also less than that of the corresponding relaxed DNA.

【 授权许可】

Unknown