Affinity labelling of the GM2‐activator protein

FEBS Letters

Sandhoff, Konrad¹  Neuenhofer, Stephan¹ 

[1] Institut für Organische Chemie und Biochemie der Universität Bonn, Gerhard-Domagk-Straβe 1, D-5300 Bonn I, FRG

关键词: Ganglioside;    Hexosaminidase;    Lysoganglioside GM2;    Bromoacetyllysoganglioside GM2;    gm2;    ganglioside GM2;    II3Neu5Ac-GgOse3Cer;    monosialogangliotriaosylceramide;    lyso-GM2;    II3Neu5Ac-GgOse3Sph;    monosialogangliotriaosylsphingosine;    GM3;    ganglioside GM3;    II3 Neu5Ac-LacCer;    monosialolactosylceramide;    lyso-GM3;    II3Neu5AcLac Sph;    monosialolactosylsphingosine;    NAc-lyso-GM2(3);    N-acetyllyso-GM2(3);    NBrAc-lyso-GM2(3);    N-bromoacetyllyso-GM2(3);    MUGlcNac;    4-methylumbelliferyl-β-D-N-acetylglucosaminid;    MUGalNac-6-SO4;    4-methylumbelliferyl-β-D-N-acetylgalactosaminid-6-sulfate;    Hex A;    hexosaminidase A;    β-2-acetamido-2-desoxy-D-glucosid: acetamidodesoxyglucohydrolase;    EC 3.2.1.30;    tlc;    thin-layer chromatography;   

DOI  :  10.1016/0014-5793(85)80751-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In contrast to ganglioside G_M2 (G_M2), its fatty acid free derivative, lyso-G_M2, and N-acetyllyso-G_M2 are hydrolyzed by purified human hexosaminidase A (Hex A). The addition of G_M2-activator stimulates the Hex A-catalyzed hydrolysis of G_M2 and that of N-acetyllyso-G_M2 much more than that of lyso-G_M2. Based on this observation N-brornoacetyllyso-G_M2 was synthesized as a potential affinity ligand of the G_M2-activator protein. N-Bromo[³H]acetyllyso-G_M2 inactivated the G_M2-activator selectively and irreversibly by covalent modification.

【 授权许可】

Unknown