Relaxation of PvuII recognition sequence

FEBS Letters

Sayadi, Sami¹  Nasri, Moncef¹  Thomas, Daniel¹ 

[1] Laboratoire de Technologie Enzymatique, UA no. 523 du CNRS, BP 233, UTC, 60206 Compiegne Cédex, France

关键词: Restriction endonuclease;    Recognition sequence;    Specificity relaxation;    bp;    base pairs;    DMSO;    dimethyl sulfoxide;   

DOI  :  10.1016/0014-5793(85)80748-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The substrate specificity of PvuII endonuclease is relaxed in the presence of dimethyl sulfoxide. The new recognition sequences cleaved in pBR322 DNA have been found to be CCGCTG, CATCTG, CAGATG, CAGGTG and CAGCGG.

【 授权许可】

Unknown