Murine mammary FM3A carcinoma cells transformed with the herpes simplex virus type 1 thymidine kinase gene are highly sensitive to the growth‐inhibitory properties of (E)‐5‐(2‐bromovinyl)‐2'‐deoxyuridine and related compounds

FEBS Letters

Balzarini, J.¹  de Clercq, E.¹  Ayusawa, D.¹  Seno, T.¹ 

[1] Rega Institute for Medical Research, Katholieke Universiteit Lernen, Minderbroedersstraat 10, B-3000 Leuven, Belgium

关键词: Murine mammary FM3A carcinoma;    Thymidine kinase gene;    Herpes simplex virus type I;    Cytostatic activity;    (E)-5-(2-Bromovinyl)-2'-deoxyuridine;   

DOI  :  10.1016/0014-5793(85)80747-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Murine mammary carcinoma (FM3A TK⁻/HSV-1 TK⁺) cells, which are thymidine kinase (TK)-deficient but have been transformed with the herpes simplex virus type 1 (HSV-1) TK gene are inhibited in their growth by (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU), (E)-5-(2-iodovinyl)-2'-deoxyuridine (IVDU) and (E)-5-(2-bromovinyl)-2'-deoxycytidine (BVDC) at 0.5, 0.5 and 0.8 , respectively; i.e., a concentration 5000 to 20000-fold lower than that required to inhibit the growth of the corresponding wild-type FM3A/0 cells. Hence, transformation of tumor cells with the HSV-1 TK gene makes them particularly sensitive to the cytostatic action ofBVDU and related compounds.

【 授权许可】

Unknown