Appearance of an arachidonic acid 15‐lipoxygenase pathway upon differentiation of the human promyelocytic cell‐line HL‐60

FEBS Letters

Samuelsson, Bengt¹  Lundberg, Ulf¹  Serhan, Charles N.¹ 

[1] Department of Physiological Chemistry, Karolinska Institutet, S-104 01 Stockholm, Sweden

关键词: Arachidonic acid;    Leukotriene;    15-Lipoxygenase pathway;    15-HPETE;    15(S)-hydroperoxy-5;    8;    11;    13-eicosatetraenoic acid;    15-HETE;    15(S)-hydroxy-5;    8;    11;    13-eicosatetraenoic acid;    5-HETE;    5(S)-hydroxy-6;    8;    11;    14-eicosatetraenoic acid;    12-HETE;    12(S)-hydroxy-5;    8;    10;    14-eicosatetraenoic acid;    LTB4;    5(S);    12(R)-dihydroxy-6;    14-cis-8;    10-trans-eicosatetraenoic acid;    8;    15-DHETE;    8;    15(S)-dihydroxy-5;    9;    11;    13-eicosatetraenoic acid;    14;    15-DHETE;    14(R);    15(S)-dihydroxy-5;    8;    10;    12-eicosatetraenoic acid;    DMSO;    dimethyl sulf-oxide;    GC-MS;    gas chromatography-mass spectrometry;    RP-HPLC;    reverse-phase high-performance liquid chromatography;    O2 .−;    Superoxide ion;   

DOI  :  10.1016/0014-5793(85)80731-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The metabolism of arachidonic acid and 15-HPETE was studied in a human promyelocytic cell line (HL-60). Upon exposure to DMSO, HL-60 cells undergo differentiation and acquire a 15-lipoxygenase activity while undifferentiated cells challenged with either arachidonic acid or 15-HPETE did not enzymatically transform these precursors. Products of the arachidonic acid 15-lipoxygenase pathway were identified by HPLC. UV-absorption and gas chromatography-mass spectrometry. Results indicate that upon differentiation HL-60 cells express a 15-lipoxygenase activity as well as the ability to transform 15-HPETE to 8,15-DHETEs and 14,15-DHETE. Moreover, these findings suggest that products of the 15-lipoxygenase cascade may be generated by a single cell system.

【 授权许可】

Unknown