A distinct ferredoxin for nitrogen fixation isolated from heterocysts of the cyanobacterium Anabaena variabilis

FEBS Letters

Böhme, Herbert¹  Schrautemeier, Bernhard¹ 

[1] Lehrstuhlfür Physiologie und Biochemie der Pflanzen, Fakultät für Biologie, Universität Konstanz, Postfach 5560, D-7750 Konstanz, FRG

关键词: Heterocyst Nitrogen fixation Ferredoxin Reconstitution Electron transport Anabaena variabilis;    ATCC;    American Type Culture Collection;    PS;    photosystem;   

DOI  :  10.1016/0014-5793(85)80627-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ferredoxin from heterocysts of Anabaena variabilis has been isolated and its biological activity compared to ferredoxin obtained from vegetative cells of the same organism. Both ferredoxins catalyze equally effective NADP⁺ photoreduction by heterocyst thylakoids. When photoreduced, both ferredoxins transfer electrons to nitrogenase from A. variabilis, with heterocyst ferredoxin being twice as active as vegetative cell ferredoxin. In the dark, however, only heterocyst ferredoxin is able to link reducing power, generated by soluble systems, such as H₂/hydrogenase (from Clostridium pasteurianum) and NADPH/ferredoxin:NADP⁺ oxidoreductase (from A. variabilis), to the cyanobacterial nitrogenase. Using heterocyst homogenates with glucose 6-phosphate as electron donor, only ferredoxin from heterocysts is able to stimulate nitrogenase activity further.

【 授权许可】

Unknown