The complete amino acid sequences of the β1‐ and β2‐subunits of the isolectins LoL1 and LoL11 from seeds of Lathyrus ochrus (L.) DC

FEBS Letters

Yarwood, A.²  Rougé, P.³  Sousa-Cavada, B.¹  Richardson, M.² 

[1] Department of Biochemistry, Federal University of Pelotas, Pelotas (RS Brazil;Department of Botany, University of Durham, Science Laboratories, South Road Durham DH1 3 LE, England,;Laboratoire de Botanique et Biologie Cellulaire, Faculté des Sciences Pharmaceutiques, Université Paul Sabatier, 35 chemin des Maraîchers, 31062 Toulouse, France

关键词: Lathyrus ochrus;    isolectin;    β-Subunit;    Amino;    acid;    sequence;    Homology;    Legume;    lectin;   

DOI  :  10.1016/0014-5793(85)80663-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete amino acid sequences of the β₁- and β₂-subunits of the isolectins (LoL1 and LoL11) from seeds of Lathyrus ochrus were determined by analysis of peptides derived from the proteins by digestion with trypsin, chymotrypsin, pepsin and the S. aureus V₈ protease, as well as fragments produced by cleavage with iodosobenzoic acid. Both β-subunits consisted of singlepolypeptide chains of 181 amino acids, which differed from one another in only 3 positions. The homology of the Lathyrus ochrus isolectins with the other two-chain lectins of the tribe Vicieae, and the single-chain lectins of other tribes of the Leguminosae is discussed.

【 授权许可】

Unknown