The use of carotenoids and oxonol VI as probes for membrane potential in proteoliposomes

FEBS Letters

Kraayenhof, R.¹  Krab, K.¹  van Walraven, H.S.¹  Hagendoorn, M.J.M.¹ 

[1] Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands

关键词: Proteoliposome;    Membrane potential;    Diffusion potential;    Carotenoid shift;    Oxonol VI;   

DOI  :  10.1016/0014-5793(85)80661-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelenght is obtained with valinomycin-induced potassium ion diffusion potentials, irrespective of the polarity of the potassium gradient. In contrast to this, the (externally added) probe oxonol VI only shows an absorbance band shift when the external potassium ion concentration is higher than the internal one. In liposomes without ATPase complex, no carotenoid absorbance band shifts were observed.

【 授权许可】

Unknown