Effect of temperature on the circular dichroism spectra of β2‐microglobulins

FEBS Letters

Brown, Eleanor M.¹  Groves, Merton L.¹ 

[1] Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 East Mermaid Lane, Philadelphia, PA 19118, USA

关键词: β2-Microglobulin;    Near-UV CD;    Temperature effect;    Tyrosine;    β-Turn;   

DOI  :  10.1016/0014-5793(85)80647-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When the temperature was lowered from 25 to 5°C dramatic changes were observed in the near-ultraviolet circular dichroism spectra of bovine and caprine but not human β₂-imcroglobulin. Comparison of the protein sequences suggests that the conformational change occurs in the amino-terminal 24 residues and that a tyrosine residue located on a potential β-turn acts as a reporter group. Because ΔH° is small (−22 kcal · mol⁻¹), such conformational changes, possibly not readily observed, may occur at low temperatures in other proteins having potential β-turns in otherwise aperiodic regions of sequence.

【 授权许可】

Unknown