Biosynthesis of androgens and pheromonal steroids in neonatal porcine testicular preparations

FEBS Letters

Kwan, T.K.¹  Orengo, C.¹  Gower, D.B.¹ 

[1] Department of Biochemistry, Guy's Hospital Medical School, London SE1 9RT, England

关键词: Porcine;    Neonate;    Testis;    Androgen;    16-Androstene;    3-Hydroxysteroid oxidoreductase;    androstenedione;    4-androstene-3;    17-di-one;    pregnenolone;    3β-hydroxy-5-pregnen-20-one;    17-hydroxyprogesterone;    17-hydroxy-4-pregnene-3;    20-di-one;    16-dehydropregnenolone;    3β-hydroxy-5;    16-pregnadien-20-one;    testosterone;    17β-hydroxy-4-androsten-3-one;    5α-DHT;    17β-hydroxy-5α-androstan-3-one;    andien-β;    5;    16-androstadien-3β-ol;    androstadienone;    4;    16-androstadien-3-one;    5α-androstenone;    5α-androst-16-en-3-one;    an-α-(β);    5α-androst-16-en-3α(β)-ol;    Enzyme abbreviations: C-17;    20-lyase;    17-hydroxy-C21 steroid C-17;    20-lyase;    3α-(β)-HSDHs;    3α-(β)-hydroxysteroid dehydrogenases [3α(β)-hydroxysteroid:NAD(P)+-oxidoreductases;    EC 1.1.1.50 and 51];    17β-HSDH;    17β-hydroxysteroid:NADP+17-oxidoreductase;    EC 1.1.1.64;   

DOI  :  10.1016/0014-5793(85)80810-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The biosynthesis of testosterone and 4-androstene-3,17-dione and some 16-androstenes has been studied in homogenates or subcellular fractions of testes from 3-week-old Landrace piglets. Pregnenolone was converted into 5,16-androstadien-3β-ol, 4,16-androstadien-3-one, 5α-androst-16-en-3-one and 5α-androst-16-en-3α- and 3β-ols, but the quantities were some 50 times less than those formed in the mature boar testis. Androgens were also formed in the microsomal fractions but the quantities of 4-androstene-3,17-dione (from side-chain cleavage of 17-hydroxyprogesterone) and of testosterone (from reduction of 4-andro stene-3,17-dione) were 50–70 times lower than in the adult animal. The kinetic parameters and cofactor preference of the 3α- and 3β-hydroxysteroid dehydrogenases were determined in the cytosolic, microsomal and mitochondrial fractions of neonatal porcine testes.

【 授权许可】

Unknown