Interaction of plasmid DNA with yeast protoplasts and a mechanism of genetic transformation

FEBS Letters

Brzobohatý, Břetislav¹  Kováč, Ladislav¹ 

[1] Institute of Animal Physiology, Slovak Academy of Sciences, 900 28 Ivanka pri Dunaji, Czechoslovakia

关键词: Cell fusion;    Plasmid DNA;    Transformation;    S. cerevisiae;    PEG;    polyethylene glycol 6000;   

DOI  :  10.1016/0014-5793(85)80778-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA of plasmid pBB 29 is bound to protoplasts of Saccharomyces cerevisiae by a time-dependent, energy-independent process requiring Ca²⁺. It is not degraded during binding but most of the bound DNA can be degraded by exogenously added DNase. Foreign DNA competes for the binding. Genetic transformation of protoplasts depends on the same conditions as does the binding of DNA but, in addition, it also requires conditions allowing fusion of the protoplasts. Transformation may consist of firm adsorption of plasmid DNA to the protoplast surface, mediated by Ca²⁺, and of internalization of the DNA entrapped between two aggregated protoplasts as a suit of their fusion.

【 授权许可】

Unknown