Effects of GTP,GDP[βS] and glucose on adenylate cyclase activity of E. coli B

FEBS Letters

Stein, Janet M.¹  Martin, B.R.¹  Kornberg, H.L.¹ 

[1] Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, England

关键词: E.coli;    Adenylate cyclase;    Catabolite repression;    GTP;    GTP[γS];    GDP;    GDP[βS];   

DOI  :  10.1016/0014-5793(85)80348-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Adenylate cyclase activity was measured in suspensions of E. coli B, rendered permeable with toluene. The enzyme was activated in a dose-dependent manner by GTP and by its non-hydrolysable analogue, GTP[γS]. In contrast, incubation with GDP[βS], a non-phosphorylatable analogue of GDP, caused a dose-related inhibition of adenylate cyclase; this was partially overcome by addition of GTP. GTP did not relieve, and GDP[βS] augmented, the non-competitive and dose-related inhibition of E.coli adenylate cyclase by glucose.

【 授权许可】

Unknown