Preparation and characterization of trypsin‐nicked ovotransferrin

FEBS Letters

Ikeda, Hiroshi¹  Tanaka, Yuichi¹  Nakazato, Katsuyoshi¹  Satake, Kazuo¹  Nabuchi, Yoshiaki¹ 

[1]Department of Chemistry, Faculty of Science, Science University of Tokyo, 1-3, Kagurazaka, Shinjuku-ku, Tokyo 162, Japan

关键词: Transferrin;    Limited proteolysis;    Denaturation;    Cooperativity;    HPLC;    high-performance liquid chromatography;    Tf;    ovotransferrin;    Fe2Tf;    Fe(III)-saturated Tf;    nicked Fe2Tf;    Fe(III)-saturated nicked Tf;    GuHCl;    guanidine hydrochloride;   

DOI  :  10.1016/0014-5793(85)80321-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The N- and C-domains isolated from ovotransferrin (Tf) with trypsin could be separated from each other and from intact Tf by HPLC with a TSK-GEL G-3000SWG-0.1% SDS system. The analytical method revealed that Fe (III)-saturated Tf (Fe₂Tf) of 77 kDa was hydrolyzed by trypsin preferentially at the portion connecting both domains. The main product was a nicked Fe₂Tf, in which the two fragmented domains of 35 kDa each were still bound together non-covalently and showed a notable cooperativity on their denaturation.

【 授权许可】

Unknown