Tobacco plants transformed with the Agrobacterium T‐DNA gene 1 contain high amounts of indole‐3‐acetamide

FEBS Letters

Rüdelsheim, P.¹  Van Onckelen, H.¹  Messens, E.²  Inzé, D.³  Van Montagu, M.³  Horemans, S.¹  Schell, J.³  De Greef, J.¹  Follin, A.³ 

[1]Departement voor Biologie, Universitaire Instelling Antwerpen, B-2610 Wilrijk, Belgium

[2]Histologisch Instituut, Rijksuniversiteit Gent, B-9000 Gent, Belgium

[3]Laboratorium voor Genetica, Rijksuniversileit Gent, B-9000 GentBelgium

关键词: Indole-3-acetamide;    Indole-3-acetic acid;    Agrobacterium tumefaciens;    T-DNA gene;    Nicotiana tabacum;    BHT;    2;    6-di(tert-butyl)-4-methylphenol;    IAA;    indole-3-acetic acid;    IAM;    indole-3-acetamide;    RT;    retention time;   

DOI  :  10.1016/0014-5793(85)80295-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The T-DNA genes 1 and 2 of the Ti plasmid of Agrobacterium tumefaciens are involved in the biosynthesis of IAA in transformed plant cells. Previously, it has been shown that gene 2 codes for an amidohydrolase able to convert IAM into IAA. We have isolated Nicotiana tabacum regenerates transformed with either gene 1 or genes 6a and 6b of the T-DNA. The tobacco plants transformed with gene 1 contain 500–1000-times more IAM as compared to plants transformed with genes 6a and 6b, and as compared to untransformed control plants. No drastic differences in endogenous IAA concentrations were observed between the three plant types analyzed.

【 授权许可】

Unknown