Both insulin and epidermal growth factor stimulate fatty acid synthesis and increase phosphorylation of acetyl‐CoA carboxylase and ATP‐citrate lyase in isolated hepatocytes

FEBS Letters

Hardie, D.Grahame¹  Holland, Ross¹ 

[1] MRC Protein Phosphorylation Group, Biochemistry Department, Dundee University, Medical Sciences Institute, Dundee DDI 4HN, Scotland

关键词: Insulin;    Epidermal growth factor;    ATP-citrate lyase;    Acetyl-CoA carboxylase;    Protein phosphorylation;    EGF;    epidermal growth factor;    IGF-1;    insulin-like growth factor-1;    HPLC;    high-performance liquid chromatography;    PDGF;    platelet-derived growth factor;   

DOI  :  10.1016/0014-5793(85)80282-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Insulin and EGF cause identical stimulation(≅40%) of fatty acid synthesis in hepatocytes isolated from rats which have been starved and then refed a low-fat diet. In both cases this stimulation is associated with increased phosphorylation of ATP-citrate lyase and of a specific site on acetyl-CoA carboxylase. However, the altered phosphorylation of acetyl-CoA carboxylase is not associated with a change in kinetic parameters which is detectable in the purified enzyme. Whatever the mechanism involved, stimulation of fatty acid synthesis by growth factors may have a role in providing new phospholipid for growth of membranes.

【 授权许可】

Unknown