β2‐Inhibin contains the active core of human seminal plasma β‐inhibin: synthesis and bioactivity

FEBS Letters

Sheth, A.R.²  Arbatti, N.J.³  Chrétien, M.⁴  Rochemont, J.⁴  Escher, E.¹  Seidah, N.G.⁴ 

[1] Department of Pharmacology, Centre Hospitalier Universitaire, Sherbrooke, P.Q., CanadaUSA;Institute for Research in Reproduction (ICMR), Bombay, India;Reproduction Research Laboratory at the Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, Austria;Laboratories of Biochemical and Molecular Neuroendocrinolog, the Clinical Research Institute of Montreal, 110 Pine Avenue West, ontreal, Austria

关键词: β2-Inhibin;    Chemical synthesis;    Human seminal plasma;    Pituitary FSH release;   

DOI  :  10.1016/0014-5793(85)81113-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete synthesis of the C-terminal 28 residues segment 67–94 of human seminal plasma jS-Inhibin, called β₂-Inhibin, is reported. The Inhibin-like activity of the native 94 amino acids β-Inhibin is compared to that of the synthetic replica of β₂-Inhibin. In all assays used both peptides effectively suppress the FSH release induced by LHRH but have little effect on the LH release. In the mouse both peptides are equipotent on a mole basis. In the rat the synthetic β₂-Inhibin is 3–10 times more potent than β-Inhibin. Both peptides are active in rat anterior pituitary primary culture assays where maximum suppression of FSH release induced by LHRH occurs around 300 of β₂-Inhibin. In contrast, maximum suppression of FSH release in the mouse pituitary assay occurs at 10–15 of either Inhibin.

【 授权许可】

Unknown