Prostaglandin E2‐like activity of 20:3n‐9 platelet lipoxygenase end‐product

FEBS Letters

Burtin, M.³  Renaud, S.³  Sprecher, H.²  Dechavanne, M.³  Lagarde, M.³  Rigaud, M.¹ 

[1] Laboratoire de Biochimie CHU Dupuytren, Limoges, France;Department of Physiological Chemistry, Ohio State University, Columbus, Ohio, USA;INSERM, Unit 63, 22 av. Doyen Lépine, 69675 Bron and Laboratoire d'Hémobiologie, Faculté Alexis Carrel, Lyon, France

关键词: Human platelet;    Lipoxygenase-5;    8;    11-icosatrienoic acid;    Platelet aggregation;    PG;    prostaglandin;    20:3n-9;    5;    8;    11-icosatrienoic acid;    U46619;    9-methano analogue of PGH2;    HPLC;    high-performance liquid chromatography;    TLC;    thin-layer chromatography;    GC-MS;    gas-liquid chromatography-mass spectrometry;    TMS;    trimethylsilyl;    BSTFA;    bis-silyl trifluoroacetamide;   

DOI  :  10.1016/0014-5793(85)81112-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

5,8,11-Icosatrienoic acid (20:3n-9), a fatty acid associated with platelet hyperactivity, was oxygenated by platelet lipoxygenase. The end-product of this pathway was purified by high-performance liquid chromatography (HPLC) and characterized as 12-hydroxy-5,8,10-icosatrienoic acid [12-OH-20:3(5,8,10)] by capillary gas-liquid mass spectrometry. When tested upon platelet aggregation, 12-OH-20:3(5,8,10) exhibited a biphasic effect. At low concentrations (below 5 × 10⁻⁷ M) it potentiated aggregation but inhibited it at higher levels, a pattern similar to that obtained with prostaglandin E₂. However, since the amounts of 12-OH-20:3(5,8,10) generated under thrombin stimulation are in the range of concentrations with potentiating effects, it seems that the 12-OH derivative is responsible for the hyperaggrebility of 20: 3n-9-rich platelets.

【 授权许可】

Unknown