Involvement of plastoquinone and lipids in electron transport reactions mediated by the cytochrome b 6‐f complex isolated from spinach

FEBS Letters

Chain, Richard K.¹ 

[1] Division of Molecular Plant Biology, University of California, Berkeley, CA 94720, USA

关键词: Cytochrome;    b 6 -f complex;    Plastoquinone;    Lipid;    Reconstitution;    PC;    phosphatidylcholine;    PG;    phosphatidylglycerol;    MGDG monogalactosyldiacylglycerol;    DGDG;    digalactosyldiacylglycerol;    SQDG;    sulfoquinovosyldiacylglycerol;    TLC;    thin-layer chromatography;    DNP-INT;    2;    4-dinitrophenyl ether of iodonitrothymol;    PQ;    plastoquinone;    b 6;    cytochrome b 6;    f;    cytochrome f;   

DOI  :  10.1016/0014-5793(85)81095-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The isolation of a cytochrome b ₆-f complex from spinach, which is depleted of plastoquinone (and lipid), is reported. The depleted complex no longer functions as a plastoquinol-plastocyanin oxidoreductase but can be reconstituted with plastoquinone and exogenous lipids. The lipid classes digalactosyldiacylglycerol, phosphatidylglycerol and phosphatidylcholine were active in reconstitution while monogalactosyldiacylglycerol and sulfoquinovosyldiacylglycerol were not. Neither plastoquinone nor lipid alone fully reconstitutes electron transport in the depleted complex. Saturation of plastoquinol-plastocyanin oxidoreductase activity in the depleted complex occurs at 1 plastoquinone per cytochrome f.

【 授权许可】

Unknown