Phorbol ester (TPA) potentiates noradrenaline and acetylcholine‐evoked amylase secretion in the rat pancreas

FEBS Letters

Singh, Jaipaul¹ 

[1] M.R.C. Secretory Control Research Group, Department of Physiology, University of Liverpool, PO Box 147, Liverpool, L69 3BX, England

关键词: Amylase secreation;    Rat pancreas;    Phorbol ester;    Acetylcholine;    Noradrenaline;    Cyclic AMP;   

DOI  :  10.1016/0014-5793(85)81069-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of the phorbol ester 12-O-tetradeconyl-phorbol-13-acetate (TPA) on noradrenaline (NA) and acetylcholine (ACh)-evoked amylase secretion in isolated segments of rat pancreas was investigated. TPA alone evoked a relatively small increase in amylase output. However, when combined with either noradrenaline or ACh, the phorbol ester markedly enhanced the secretagogue-induced amylase secretion. These effects were dose related. TPA also enhanced the amylase secretion evoked by either the Ca²⁺ ionophore A23187 or dibutyryl cyclic AMP. This potentiation by TPA of noradrenaline, ACh, Ca²⁺ ionophore A23187 and dibutyryl cyclic AMP-evoked amylase output may suggest the existence of a third pathway controlling enzyme secretion in the pancreas.

【 授权许可】

Unknown