Rapid proton release accompanying photosynthetic electron transport in intact cells of Rhodopseudomonas capsulata

FEBS Letters

Taylor, M.A.¹  Jackson, J.B.¹ 

[1] Department of Biochemistry, University of Birmingham, PO Box 363, Birmingham B15 2TT, England

关键词: Proton translocation;    Photosynthesis;    Electron transport;    Photosynthetic bacteria;   

DOI  :  10.1016/0014-5793(85)81060-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The release of protons from intact cells of Rhodopseudomonas capsulata after either 4μs flashes or during brief periods of continuous illumination has been measured with the indicator, cresol red. The half-time for H⁺-release after a flash was 35 ms and the extent, 1H⁺ per 134 bacteriochlorophyll. Myxothiazol completely inhibited the flash-induced H⁺-release and antimycin A reduced it by 37%. The proton-releasing reaction is discussed with reference to the protonmotive Q-cycle. During continuous illumination the rapid phase of H⁺ release is followed by a lag and then by another period of acidification, suggesting that other protolytic reactions may be in operation.

【 授权许可】

Unknown