Effect of polyethylene glycol on the kinetic behaviour of pyruvate kinase and other potentially regulatory liver enzymes

FEBS Letters

Sols, Alberto¹  Aragón, Juan J.¹  Medina, Ruth¹ 

[1] Departamento Enzimología, Instituto Investigaciones Biomédicas del CSIC, Facultad de Medicina de la UAM, 28029 Madrid, Spain

关键词: Enzyme concentration;    Polyethylene glycol;    Liver enzyme;    Pyruvate kinase;    Glycogen phosphorylase;    a;   

DOI  :  10.1016/0014-5793(85)80235-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Assay in the presence of 10% polyethylene glycol has been systematically used with potentially regulatory liver enzymes as an indirect way to induce aggregation of enzymes corresponding to that which could occur at their physiological concentrations. Pyruvate kinase L was markedly affected by polyethylene glycol, as was muscle phosphorylase a, while pyruvate kinase M as well as glucokinase, fructose-1,6-bisphosphatase and other liver enzymes examined were not affected.

【 授权许可】

Unknown