Phenylalanine ammonia‐lyase immobilized in semipermeable microcapsules for enzyme replacement in phenylketonuria

FEBS Letters

Bourget, Louis¹  Chang, Thomas M.S.¹ 

[1] Artificial Cells and Organs Research Centre, Departments of Physiology and Medicine, McGill University, 3655 Drummond Street, Montreal, Canada H3G 1 Y6

关键词: Phenylketonuria;    Artificial cell;    Microencapsulation;    Phenylalanine ammonia-lyase;    Enzyme immobilization;    Inborn errors of metabolism;    PAL;    phenylalanine ammonia-lyase;    EC 4.3.1.5;   

DOI  :  10.1016/0014-5793(85)80219-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phenylalanine ammonia-lyase immobilized within semipermeable microcapsules has an assayed enzyme activity which is 20% ± 4% of the enzyme in free solution. The K_m for the immobilized enzyme remained the same as that of the free enzyme. The pH optimum also remained unchanged at pH 8.5 ± 1.0. At the lower pH range, enzyme activity is higher for the immobilized enzyme. Daily oral administration of microencapsulated phenylalanine ammonia-lyase to phenylketonuric rats decreased the systemic phenylalanine level by 35 ± 8% in 2 days (P < 0.05) and by 75 ± 8% in 7 days (P < 0.001).

【 授权许可】

Unknown