The antigenic structure and topography of bacteriorhodopsin in purple membranes as determined by interaction with monoclonal antibodies

FEBS Letters

Vturina, I.Yu.¹  Ovchinnikov, Yu.A.¹  Kiselev, A.V.¹  Kuryatov, A.B.¹  Vasilov, R.G.¹  Abdulaev, N.G.¹ 

[1] Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow, USSR

关键词: Bacteriorhodopsin;    Monoclonal antibody;    Membrane;    Antigenic structure Enzyme-linked immunoadsorbent assay Protein;   

DOI  :  10.1016/0014-5793(85)80548-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The spatial organization and the antigenic structure of the bacteriorhodopsin molecule in the purple membrane were studied by immunochemical techniques. Five monoclonal antibodies directed against exposed parts of the protein molecule in the membrane were prepared and characterized. Antigenic determinants were localized in the bacteriorhodopsin polypeptide chain by analysis of the interaction between monoclonal antibodies and protein fragments. The structure of antigenic determinants was revealed by the interaction of monoclonal antibodies with (i) isolated bacteriorhodopsin fragments further modified by sequential Edman degradation and (ii) derivatives of bacteriorhodopsin obtained biosynthetically or by selective chemical modification. Five antigenic determinants were localized in the following parts of bacteriorhodopsin: < Glu'-Met²⁰ involving one of the 3 amino acid residues of the N-terminal part; Gly³³-Met⁵⁶ involving Asp³⁶ and/or Asp³⁸ and Phe⁴²; Phe¹⁵⁶-Met¹⁶³ involving Phe¹⁵⁶; Glu¹⁹⁴-Leu²⁰⁷ involving Glu¹⁹⁴; Pro²⁰⁰-Leu²⁰⁷.

【 授权许可】

Unknown