An abundant ubiquitous glycoprotein (GP100) in nucleated mammalian cells

FEBS Letters

Mortara, R.A.¹  Smith, M.J.¹  Koch, G.L.E.¹ 

[1] Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, England

关键词: Con A receptor glycoprotein;    Abundant glycoprotein;    GP100;    Common glycoprotein Nucleated cell glycoprotein;    Membrane structure;    Con A;    concanavalin A;    PMSF;    phenylmethylsulfonyl fluoride;    TLCK;    p-tosyl lysyl chloroketone;   

DOI  :  10.1016/0014-5793(85)80537-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two-dimensional gel electrophoresis with the ¹²⁵I-Con A overlay and affinity purification with Con A-agarose revealed the presence of an abundant ubiquitous 100-kDa glycoprotein (GP₁₀₀) in nucleated mammalin cells. The amount in cultured human and murine cells varies from 3 to 20 × 10⁶ molecules per cell making GP₁₀₀ the most abundant glycoprotein in nucleated cells. Peptide mapping shows that it is different from erythrocyte Band III protein. Several properties of GP₁₀₀ suggest that it could play a structural role in nucleated cell membranes.

【 授权许可】

Unknown