tRNA binding to programmed ribosomes increases the ribosomal affinity for tuberactinomycin O

FEBS Letters

Yamada, Takeshi¹  Nierhaus, Knud H.³  Shiba, Tetsuo²  Teshima, Tadashi² 

[1]Research Institute for Microbial Diseases, Osaka University, Yamadaoka, 3-1, Suita City, Osaka 565, Japan

[2]Department of Chemistry, Faculty of Science, Osaka University, Toyonaka, Osaka 560, Japan

[3]Abt. Wittmann, Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 63-73, 1000 Berlin 33 (Dahlem), Germany

关键词: Tuberactinomycin O binding;    Protein synthesis inhibitor;    Equilibrium dialysis Ribosomal tRNA binding;    Antibiotic;   

DOI  :  10.1016/0014-5793(85)80186-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding of ¹⁴C-labelled tuberactinomycin O was analysed in equilibrium dialysis cells. The ionic conditions and the concentration of the labelled drug used in the binding assays allowed the binding of just one drug molecule per non-programmed ribosome. Under these conditions, the occupation of the ribosomal P-site by deacylated tRNA^Phe in the presence of poly(U) increased the amount of [¹⁴C]tuberactinomycin O bound by a factor of two. Kanamycin, gentamicin and neomycin reduced the binding of tuberactinomycin O, whereas chloramphenicol, tetracycline, streptomycin and puromycin had no effect. A stimulation of the binding of tuberactinomycin O was found upon addition of erythromycin.

【 授权许可】

Unknown