Inhibition of the activity of restriction endonucleases by spermidine and spermine

FEBS Letters

Kuosmanen, Malla¹  Pösö, Hannu¹ 

[1] Department of Pharmacology and Toxicology, College of Veterinary Medicine, PO Box 6, Hämeentie 57, SF-00551, Helsinki 55, Finland

关键词: Endonuclease;    Polyamine;    Plasmid digestion inhibition;   

DOI  :  10.1016/0014-5793(85)80181-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Physiological concentrations (0.5–2.0 mM) of spermidine and spermine were observed to inhibit the digestion in vitro of plasmid pJDB 207 by the restriction endonucleases BamHI (EC 3.1.23.6), EcoRI (EC 3.2.23.13), HindIII (EC 3.1.23.20), HpaI (EC 3.1.23.23) and PstI (EC 3.1.23.31). The polyamines protected all the tested restriction sequences of DNA, since the activity of all endonucleases used was strongly inhibited. These results show the need for caution when using polyamines as experimental tools for recombinant DNA chemistry.

【 授权许可】

Unknown