Inhibition of glutathione efflux in the recirculating rat liver perfusion by cysteine but not by oxothiazolidine carboxylate, an intracellular cysteine precursor

FEBS Letters

Glazenburg, Eitjo J.¹  Mulder, Gerard J.²  Bruggink, Jan E.¹  Wolters-Keulemans, Katja¹ 

[1] Department of Pharmacology, State University of Groningen, Bloemsingel 1, 7913 BZ Groningen, The Netherlands;Division of Toxicology, Center for Bio-Pharmaceutical Sciences, University of Leiden. PO Box 9503, 2300 RA Leiden, The Netherlands

关键词: Cysteine;    Cystine;    Glutathione;    Liver perfusion;    Oxothiazolidine carboxylate;    Sulfate;   

DOI  :  10.1016/0014-5793(84)80614-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the recirculating rat liver perfusion a continuous release of glutathione into the perfusion medium is observed. Addition of L-cysteine to the perfusion medium immediately arrested this glutathione efflex. The cysteine precursor oxothiazolidine carboxylate did not block the glutathione efflux in spite of the fact that it generated more L-cysteine inside the liver cells than L-cysteine itself; L-cysteine is rapidly oxidized to cystine, that is no longer taken up by the liver. The results suggest that the inhibition of glutathione efflux results from the presence of cystine in the perfusion medium.

【 授权许可】

Unknown